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seamless cloning kit  (Beyotime)


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    Structured Review

    Beyotime seamless cloning kit
    Seamless Cloning Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/seamless+cloning+kit/Seamless+Cloning+Kit/pmc13054033-109-18-21
    Average 99 stars, based on 454 article reviews
    seamless cloning kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334- shdA . ..

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance.
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334-shdA. ..

    Polymerase Chain Reaction:

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334- shdA . ..

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance.
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334-shdA. ..

    Plasmid Preparation:

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334- shdA . ..

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance.
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334-shdA. ..

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae.
    Article Snippet: .. Full- length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Article Title: Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence in Acinetobacter baumannii
    Article Snippet: For complementation, the full-length target gene from AB5116 and linearized pAT03 were amplified using PrimeSTAR Max DNA polymerase (TaKaRa). .. The target gene was cloned into pAT03 using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China), and the recombinant plasmid was transformed into DH5α competent cells. ..

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae
    Article Snippet: .. Full‐length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Cloning:

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334- shdA . ..

    Article Title: Integrated ATAC-Seq and RNA-Seq Analyses Identify the Motif CGTTTCCGGT as an Arginine Deficiency-Responsive DNA Element in Cancer Cells.
    Article Snippet: .. The amplified fragment was cloned into pGL3-vector using the Seamless Cloning Kit (Beyotime). .. The resulting construct was verified by Sanger sequencing (Genewiz, Suzhou, China) to confirm correct insertion and orientation.

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance.
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334-shdA. ..

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae.
    Article Snippet: .. Full- length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Article Title: Multiple Pathway Engineering Combined with Tolerance Engineering Promotes High-Level Acetoin Biosynthesis in Bacillus subtilis
    Article Snippet: Acetoin is a natural flavor compound widely present in foods, but its cytotoxicity limits microbial production.. Here, a multi-level metabolic engineering strategy was developed in Bacillus subtilis to reconstruct the acetoin pathway.. Expression of key genes in the phosphotransferase system and glycolysis was enhanced, and pyruvate overflow was blocked to direct carbon flux toward acetoin precursors.

    Article Title: Transposon mutant library reveals the complex regulatory network of biofilm formation in Vibrio parahaemolyticus
    Article Snippet: .. According to the instructions of the seamless cloning kit (Beyotime, Shanghai, China), the expression vector pET28a was digested with Bam HI and Eco RI. ..

    Article Title: Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence in Acinetobacter baumannii
    Article Snippet: For complementation, the full-length target gene from AB5116 and linearized pAT03 were amplified using PrimeSTAR Max DNA polymerase (TaKaRa). .. The target gene was cloned into pAT03 using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China), and the recombinant plasmid was transformed into DH5α competent cells. ..

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae
    Article Snippet: .. Full‐length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Recombinant:

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334- shdA . ..

    Article Title: Tail spike protein evolution enables phage Psm140 to target Salmonella ShdA and overcome host resistance.
    Article Snippet: .. The purified PCR fragments containing shdA and pYA3334 homologous arms were ligated into the linearized vector using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China, D7010M), generating the recombinant plasmid pYA3334-shdA. ..

    Article Title: Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence in Acinetobacter baumannii
    Article Snippet: For complementation, the full-length target gene from AB5116 and linearized pAT03 were amplified using PrimeSTAR Max DNA polymerase (TaKaRa). .. The target gene was cloned into pAT03 using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China), and the recombinant plasmid was transformed into DH5α competent cells. ..

    Amplification:

    Article Title: Integrated ATAC-Seq and RNA-Seq Analyses Identify the Motif CGTTTCCGGT as an Arginine Deficiency-Responsive DNA Element in Cancer Cells.
    Article Snippet: .. The amplified fragment was cloned into pGL3-vector using the Seamless Cloning Kit (Beyotime). .. The resulting construct was verified by Sanger sequencing (Genewiz, Suzhou, China) to confirm correct insertion and orientation.

    Clone Assay:

    Article Title: Integrated ATAC-Seq and RNA-Seq Analyses Identify the Motif CGTTTCCGGT as an Arginine Deficiency-Responsive DNA Element in Cancer Cells.
    Article Snippet: .. The amplified fragment was cloned into pGL3-vector using the Seamless Cloning Kit (Beyotime). .. The resulting construct was verified by Sanger sequencing (Genewiz, Suzhou, China) to confirm correct insertion and orientation.

    Article Title: Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence in Acinetobacter baumannii
    Article Snippet: For complementation, the full-length target gene from AB5116 and linearized pAT03 were amplified using PrimeSTAR Max DNA polymerase (TaKaRa). .. The target gene was cloned into pAT03 using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China), and the recombinant plasmid was transformed into DH5α competent cells. ..

    Construct:

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae.
    Article Snippet: .. Full- length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae
    Article Snippet: .. Full‐length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Virus:

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae.
    Article Snippet: .. Full- length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Article Title: The Genome of the Rice Variety Mowanggu Provides Insight Into Resistance to Magnaporthe oryzae
    Article Snippet: .. Full‐length DNA fragments of target genes were constructed into a linearized pSuper1307 vector (digested with BamHI and SalI) between a cauliflower mosaic virus 35S promoter and green fluorescent protein (GFP) tag using the Seamless Cloning Kit (Beyotime). ..

    Expressing:

    Article Title: Transposon mutant library reveals the complex regulatory network of biofilm formation in Vibrio parahaemolyticus
    Article Snippet: .. According to the instructions of the seamless cloning kit (Beyotime, Shanghai, China), the expression vector pET28a was digested with Bam HI and Eco RI. ..

    Transformation Assay:

    Article Title: Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence in Acinetobacter baumannii
    Article Snippet: For complementation, the full-length target gene from AB5116 and linearized pAT03 were amplified using PrimeSTAR Max DNA polymerase (TaKaRa). .. The target gene was cloned into pAT03 using a seamless cloning kit (Beyotime Biotechnology, Shanghai, China), and the recombinant plasmid was transformed into DH5α competent cells. ..



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